Wu Na
Shengjing Hospital of China Medical Universtiy, China
Title: Acute blood glucose fluctuation enhances apoptosis of rat vascular endothelial cell in vivo
Biography
Biography: Wu Na
Abstract
Statement of the Problem: Compared to persistent hyperglycemia, fluctuant hyperglycemia has more potential to increase microvascular lesions and the risk of death. In the condition of stress, the glucose levels of those with normal metabolic function may be very high. In this case given hypoglycemic therapy, there may be glucose decreases sharply in the hypoglycemic process, leading to acute glucose fluctuations. Chronic hyperglycemia in vivo and in vitro studies showed that fluctuant hyperglycemia could increase the apoptosis of endothelial cells. But to date, few studies have been conducted to investigate the influence of acute fluctuant hyperglycemia on endothelial cells in vivo. Methodology & Theoretical Orientation: In the present study, an in vivo model of acute fluctuant hyperglycemia was successfully established. We examin the influence of acute fluctuant hyperglycemia and persistent hyperglycemia on vascular endothelial cell apoptosis, oxidative stress and inflammation in vivo. Rats were assigned to three different groups (n=8/group) that received 48-h infusions of saline (SAL group), continuous 50% glucose (constant high glucose group [CHG]), or intermittent 50% glucose (acute blood glucose fluctuation group [AFG]). Expression of related protein and mRNAs were measured in endothelial homogenates prepared from endothelial cells harvested from the aorta Findings: Endothelial cells apoptosis were observed significantly in the aortas of the AFG group. The AFG had reduced Bcl-2 levels and enhanced Bax mitochondrial translocation levels in comparison with the CHG group (P <0.05). Compared with SAL and CHG, AFG increased MDA and 8-isoprostaglandin levels in plasma, oxidative stress in vascular endothelial cells, and inflammatory cytokines in plasma and vascular endothelial cells (P <0.05). Conclusion & Significance:Acute glucose fluctuation could cause significant oxidative stress and inflammation in endothelial cells, and elevate endothelial cell apoptosis, resulting in severe cardiovascular injury. Therefore, not only lowering blood glucose, but also reducing glucose fluctuation is very important in clinic.
Recent Publications
1. Wu N, Shen H, Liu H, et al.(2016) Acute blood glucose fluctuation enhances rat aorta endothelial cell apoptosis, oxidative stress and pro-inflammatory cytokine expression in vivo. Cardiovascular Diabetology, 15:109.
2. Wu N, Lu Y, He B, et al. (2010) Taurine prevents free fatty acid-induced hepatic insulin resistance in association with inhibiting JNK1 activation and improving insulin signaling in vivo. Diabetes Research & Clinical Practice, 90:288-96.
3. Shen H, Wu N, Liu Z, et al. (2017) Epigallocatechin-3-gallate alleviates paraquat-induced acute lung injury and inhibits upregulation of toll-like receptors[J]. Life Sciences, 170:25-32.
4. Shen H, Wu N, Wang Y, et al. (2017) Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis.[J]. International Immunopharmacology, 46:16.
5. Shen H, Wu N, Wang Y, et al. (2017) MyD88 gene knockout attenuates paraquat-induced acute lung injury.[J]. Toxicology Letters, 269:41